4 Fertility Testing ELISA kits For Mothers
Women looking to have children go
through depressing moments. Knowing that you might not be able to have a child/
children when you really want to is heartbreaking; however, you should not lose
hope because your fertility specialist or gynecologist can carry out tests that
could help in sorting out the problem. Getting pregnant is always interplay of
hormones and your body not cooperating might be one of the reasons for being
unable to conceive.
Elaborate tests carried out can
pinpoint the issue and you might eventually get a child. Enzyme-linked
immunoassays are elaborate and specific and can detect the slightest change in
proteins associated with specific fertility issues.
1. E Elisa Kits
Also called Estrogen ELISA kits, this
is a test kit that uses the competitive format to determine the amount of
estrogen and its presence as well. Estrogen is a primary sex hormone present
naturally but can also be found in oral contraceptives and in hormone
replacement therapy for postmenopausal and transgender women.
In a competitively inhibited
environment, estrogen is conjugated with horseradish peroxide. The more the
estrogen in the sample the less the antibody bound to horseradish. These ELISA
test kits are readily available for scientists upon ordering online or through
making calls.
2.
Hepc25 ELISA kits
Human
Hepcidin 25, also called Hepc25, is found in serum and plasma. It exists as an
amino acid and is encoded by HAMP gene.
It regulates entry of iron into circulation by reducing iron transport
across the gut mucosa. It also reduces extrusion of macrophages by reducing
ferroportin, the iron transporter. The
levels are high when the body is under abnormally high inflammatory conditions
and could indicate a problem in pregnant mothers when the body launches an
autoimmune response against the developing fetus.
It
also utilizes the competitive ELISA assay, increasing its sensitivity .Hepc25
is bound to the immobilized body then a biotin conjugated antibody is added to
the wells. Avidin conjugated horseradish peroxide is then added after washing.
Color develops in proportion to amount of bound Hepc25.
3. Estradiol EIA kit
This ELISA test kit assays body fluid
plasma levels of 17beta-Estardiol are used to assay for fertility. Estradiol is
considered the most potent form of estrogen and is responsible for
reproduction, development of secondary female characteristics in females,
breast growth and maturation of long bones. Using an immunosorbent competitive
assay, enzyme conjugate and estradiol in the sample compete for binding sites
on the antibody-coated plate. Extent of color development is inversely
proportional to estradiol levels.
4. Zearalanone-HRP
Another protein tested in ELISA assays
to determine the cause of abortion and infertility in females, especially in
swine. Zearalanone is a mycotoxin that causes these problems. It is detected in
competitive immunoassays and scientists say that the research is promising and
could be exclusively used in humans soon.
More research is being done by
scientists to determine causes of infertility and in the future, there will be
advanced and faster ways of diagnosing infertility and reversing the problem.
These scientific processes are very important to ensure that mothers have
children when they want to and also promote surrogacy in permanently
irreversible instances.
ELISA assay kits are easily available
to scientists, customized to the species under consideration and only prepared
when the order is made. This gives you more value for your money.
Rachael Peter is a certified medical
officer who has over 7 years in the field of medicine. She has used Elisa test kits
severally when attending many mothers. She has written intensively on maters to
do with pregnancy. Visit her blog for more information.